Fig. 5. Phosphorylation of AKT by THC during oocyte maturation. Immature GV oocytes from from (A) wild type mice, (B) Cnr1^-/- mice, (C) Cnr2^-/- mice and (D) Cnr1^-/-/Cnr2^-/- mice were cultured in vitro in presence of 10-7 M of THC or in absence of it (control) for 17 h and the phosphorylation status of AKT (pAKT) was observed at 0, 10 min, 30 min, 1 h and 17 h. pAKT is shown in green. Hoechst-labelled DNA is shown in blue. n = 5 independent experiments of 15 oocytes per treatment. All of analysed oocytes had same staining pattern; representative photomicrographs are shown. Scale bars, 20 µm.